Clone | 5.8HK |
Isotype | IgG2b |
Product Type | Single-Color Reagent |
Units | 100 Tests |
Host | Mouse |
Species reactivity | Human |
Application | Flow Cytometry |
BackgroundIdentification of human B cells associated with approximately 10% of peripheral blood lymphocytes.
Synonyms: CD22 PE
Product
Product Form: RPE
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purification Method: Protein A/G Chromatography
Concentration: Titered for flow cytometry
ApplicationsPBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC.Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood.Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Functional Analysis: Flow Cytometry Staining
StorageProduct should be stored at 4-8ºC. DO NOT FREEZE
Product Stability: Reagents are stable for the period shown on the vial label when stored properly
Shipping Conditions: Room Temperature
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1.In vivo and in vitro expression of myeloid antigens on B-lineage acute lymphoblastic leukemia cells.Leukemia 1991 Ja;5(1):1925 Hara J; Kawa-Ha K; Yumura-Yagi K; Kurahashi H; Tawa A; Ishihara S; Inoue M; Murayama N; Okada S 2.Activation of dense human tonsilar B cells. Induction of c-myc gene expression via two distinct signal transduction pathways. J Immunol 1991 Feb;146(3):846-53 White MW; McConnell F; Shu GL; Morris DR; Clark EA 3.Differential effects of low and high concentrations of interleukin 6 on human B cells.Eur J Immunol 1990 No;20(11):2389-93 Levy Y; Fermand JP; Brouet JC 4.Immunophenotypes in 'classical' B-cell chronic lymphocytic leukemia. Correlation with normal cellular counterpart and clinical findings. Cancer 1990 Oct 1;66(8):1738-42 Baldini L; Cro L; Cortelezzi A; Calori R; Nobili L; Maiolo AT; Polli EE 5.B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny. Blood 1990 Oct 1;76(8):1647-56 Small TN; Keever CA; Weiner-Fedus S; Heller G; O'Reilly RJ; Flomenberg N 6.Phenotypic analysis of a large number of normal human bone marrow sample by flow cytometry. Blut 1990 No;61(5):271-7 Andreoni C; Rigal D; Bonnard M; Bernaud J 7.Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry. Blood 1990 Nov ;76(9):1739-47 Terstappen LW; Johnsen S; Segers-Nolten IM; Loken MR.
Protein Reference(s)
Database Name: UniProt
Accession number: P20273
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
Nordicbiosite
北欧生物技术公司已经经营了近20年,被认为是研究和诊断、免疫学和分子生物学、制药、生物技术、诊断和学术研究人员产品供应方面的领先企业。
Now in business for nearly 20 years, Nordic BioSite is recognized as a leader in supply of products for research and diagnostic, Immunology and Molecular Biology, to Pharmaceutical, Biotech, Diagnostic and to academic researchers.
Continuously, Nordic BioSite introduces many novel products as a result of the research and development with our partners and the feed back from our customers. Through the development, manufacturing, marketing and distribution of our products our aim is to facilitate your research.
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