BackgroundCaspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as AlzheimerÕs disease (5).

Synonyms: ICH-2, ICE(rel)-II , Mih1 (IN)

Source

Immunogen: Rabbit polyclonal Caspase-4 antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

ApplicationsCasp-4 (NT) antibody can be used for the detection of Caspase-4 by Western blot at 1 ug/ml. Depending on cell lines or tissues used, other cleavage products may be observed.Antibody can also be used for immunohistochemistry (2-4 µg/ml starting dilution).Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases.Cell 2004; 117:561-742) Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-33) Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.4) Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1-converting enzyme family of cysteine proteases.J. Biol. Chem. 1995; 270:15250-6.

Protein Reference(s)

Database Name: UniProt

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