Isotype | IgG |
Product Type | Polyclonal Antibody |
Units | 100 µg |
Host | Rabbit |
Species reactivity | HumanMouseRat |
Application | Western Blotting |
BackgroundAccumulation of the amyloid-peptide (A ) in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. The amyloid protein precursor (APP) is cleaved by secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for secretase to generate the 4 kDa amyloid-peptide (A ), which is deposited in the brains of all suffers of AlzheimerÕs disease.
Synonyms: Rabbit anti-β-Amyloid Peptide Precursor (Amyloid-β Peptide N-terminal)
Source
Immunogen: Synthetic peptide corresponding to amino acids 653 to 662 of human amyloid A4 protein precursor (APP) or 1 to 10 of the 4kDa Amyloid-peptide generated by and-secretases.This sequence is identical to those of rabbit, pig, bovine, guinea pig and chicken.
Product
Product Form: Unconjugated
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
ApplicationsDetects APP and Ab peptide by Western blot at 0.5 to 1 µg/ml.Detects an approximately 120 kDa band in human, mouse and rat brain tissue lysates.Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Murine brain lysate
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. Ponte, P., et al.A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988, 331, 525-527.2. Selkoe, D.J. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu. Rev. Cell Biol. 1994, 10, 373-403
Protein Reference(s)
Database Name: SwissProt
Accession number: P05067
Species Accession: Human
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
Nordicbiosite
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